NEUROD2 Antibody

• Catalog Number: orb2635660
• Category: Antibodies
• Description: Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix(bHLH) transcription factorsthat function as dominant negative regulators and are involved in cell lineage determination and differentiation. Neuro D2 (neurogenic differentiation 2), also known as NDRF, NEUROD2 or bHLHa1, is a 382 amino acid nuclear protein that contains one bHLH domain and functions to induce neurogenic differentiation, playing an important role in the maintenance and determination of cell fate.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-NEUROD2-1G1
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human NEUROD2 protein was used as the immunogen for the NEUROD2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This NEUROD2 antibody is available for research use only.
• UniProt ID: Q15784
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the NEUROD2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free NEUROD2 antibody (clone PCRP-NEUROD2-1G1) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using NEUROD2 antibody (green, clone PCRP-NEUROD2-1G1) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with NEUROD2 antibody (blue, clone PCRP-NEUROD2-1G1) and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using NEUROD2 antibody (clone PCRP-NEUROD2-1G1). These results demonstrate the foremost specificity of the PCRP-NEUROD2-1G1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.