Neu (phospho Tyr877) rabbit pAb

• Catalog Number: orb764239
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Neu (phospho-Tyr877)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr877. AA range:851-900
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 180
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ERBB2 antibody, anti-HER2 antibody, anti-MLN1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Neu (Y877) Polyclonal Antibody diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr877) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr877) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells and HeLa cells, using HER2 (Phospho-Tyr877) Antibody. The lane on the right is blocked with the phospho peptide.