Nestin antibodyCustom conjugation service available

Catalog Number: orb608105

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CategoryPrimary antibodies
Catalog Numberorb608105
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, IHC-P, WB
ReactivityRat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from the region aa51-130 of mouse Nestin
Concentration- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Dilution rangeWB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:100-500, ICC: 1:100-500, IF: 1:100-500
Form/AppearanceLiquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Entrez5655
ConjugationUnconjugated
MW31 kDa
TargetNestin
UniProt IDO43240 (Human)
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
DescriptionRabbit polyclonal antibody to Nestin
Alternative namesKallikrein 10, Breast normal epithelial cell associated serine protease, Kallikrein related peptidase 10, KLK 10, KLK10, KLK10_HUMAN, NES 1, NES1, Normal epithelial cell specific 1, Protease serine like 1, PRSS L1, PRSSL 1, PRSSL1.(click to expand)
Expiration Date12 months from date of receipt.
NoteFor research use only.
PurityAffinity purified by Protein A
  1. 11 images
    • IHC-P staining of rat bone marrow tissue using Nestin antibody (2.5 ug/ml)
    • Immunohistochemical staining of rat brain tissue using Nestin antibody (5 ug/ml)
    • IHC-P staining of rat pancreas tissue using anti-Nestin (2.5 ug/ml)
    • IHC-P image of rat brain tissue using anti-Nestin (5 ug/ml)
    • IHC-P image of rat kidney tissue using Nestin antibody (5 ug/ml)
    • IHC-P staining of rat kidney tissue using Nestin antibody (2.5 ug/ml)
    • IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)
    • IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)
    • Immunohistochemical staining of paraffin embedded rat kidney tissue using Nestin antibody (5 ug/ml)
    • Western blot analysis of U251 cells using Nestin antibody (3 ug/ml)
    • Immunohistochemical staining of mouse bone marrow tissue using anti-Nestin (2.5 ug/ml)
    Nestin antibody [orb11109] Custom conjugation service available

    ELISA, IHC-P, WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 200 μg
  2. 6 images
    • Immunofluorescense analysis of human bone osteosarcoma cell line using Nestin antibody (dilution at 1:25)
    • Immunofluorescense analysis of SH-SY5Y cells  using Nestin antibody (dilution at 1:200)
    • Western blot analysis of Nestin antibody in HepG2 cell line lysates
    • Immunohistrochemical analysis of formalin-fixed and paraffin-embedded human hepatocarcinoma reacted tissue using Nestin antibody
    • WB analysis of Hela whole cell lysate Lysates using Nestin antibody
    Nestin antibody [orb33615]

    ELISA, FACS, ICC, IF, IHC-P, WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    400 μl
  3. 6 images
    • Immunofluorescence image of rat brain tissue using anti-Nestin (2.5 ug/ml)
    • IHC-P image of rat brain tissue using anti-Nestin (2.5 ug/ml)
    • Immunohistochemical staining of mouse kidney tissue using Nestin antibody (2.5 ug/ml)
    • Immunohistochemical staining of rat brain tissue using Nestin antibody (2.5 ug/ml)
    • Immunofluorescence analysis of rat brain tissue using anti-Nestin (2.5 ug/ml)
    • IHC-P staining of mouse kidney tissue using anti-Nestin (2.5 ug/ml)
    Nestin antibody [orb251614] Custom conjugation service available

    ELISA, ICC, IF, IHC-P

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 200 μg
  4. 4 images
    • Immunohistochemical staining of at 40X (left) and 20X (right) Tissue: Brain and Cerebellum (right) using Nestin antibody
    • Immunohistochemical staining of mouse kidney (Left) and human kidney (Right) tissue using Nestin antibody
    • Western blot analysis of human Nestin tissue (arrowhead) using Nestin antibody
    • Western blot analysis of mouse Nestin tissue (arrowhead) using Nestin antibody
    Nestin antibody [orb345408]

    ELISA, WB, IHC

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  5. 4 images
    • Immunohistochemical staining of mouse kidney (Left) and human kidney (Right). Tissue using Nestin antibody
    • Immunohistochemical staining of at 40X (left) and 20X (right) Tissue: Brain and Cerebellum (right) using Nestin antibody
    • Western blot analysis of mouse Nestin tissue (arrowhead) using Nestin antibody
    • Western blot analysis of human Nestin tissue (arrowhead) using Nestin antibody
    Nestin antibody [orb345409]

    ELISA, WB, IHC

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    25 μl