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Catalog Number | orb20343 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NEFM |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 102; 59.5 |
Target | NEFM / NF-M |
Entrez | 4741 |
Protein Sequence | QASHITVERKDYLK |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti NEFM antibody, anti neurofilament, medium pol Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20343 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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