NDUFA7 Antibody

• Catalog Number: orb1248642
• Category: Antibodies
• Description: NDUFA7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-PKLPVGPSHKLSNN
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 15 kDa
• Target: NDUFA7
• UniProt ID: O95182
• NCBI: NP_004992.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NDUFA7, NADH dehydrogenase (ubiquinone) 1 alpha su
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 15kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 12.6kDa according to NP_004992.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Heart shows mitochondrial staining in cardial fibres. Recommended concentration: 4-8ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248642 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248642 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248642 (0.1 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.