NCOA7 Antibody (N-term)

• Catalog Number: orb1788048
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 76-103 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 106162 Da
• Target: This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
• UniProt ID: Q8NI08
• NCBI: NP_001186548.1, NP_861447.3, NP_001186551.1, NP_001116314.1, NP_001186549.1, NP_001186550.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nuclear receptor coactivator 7, 140 kDa estrogen r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-NCOA7 Antibody (N-term) at 1:1000 dilution. Lane 1: MDA-MB-453 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: mouse kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NCOA7 Antibody (N-term) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HUVEC whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.