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Catalog Number | orb1938463 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB14847 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 62943 Da |
Target | This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human NARS. |
UniProt ID | O43776 |
NCBI | NP_004530.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Asparagine--tRNA ligase, cytoplasmic, Asparaginyl- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
NARS Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
NARS Antibody (N-term) western blot analysis in WiDr, HepG2 cell line lysates (35 ug/lane). This demonstrates the NARS antibody detected the NARS protein (arrow).
Western blot analysis of NARS (arrow) using rabbit polyclonal NARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.