MRPL12 monoclonal antibody (M01), clone 3B12-1A3

• Catalog Number: orb2292245
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant MRPL12.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3B12-1A3
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE
• NCBI: AAH02344
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged MRPL12 is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3 ug/ml]

MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Western Blot analysis of MRPL12 expression in COLO 320 HSR.

Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3. Lane 1: MRPL12 transfected lysate (21.3 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # orb2292245). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (47.52 KDa).