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Catalog Number | orb345522 |
---|---|
Category | Antibodies |
Description | Mre11 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 68-706 of mouse Mre11 protein. |
Concentration | 1.05 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against mouse Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Mre11 protein from rat (94% homology). Based on protein sequence homology also expect partial reactivity against Mre11 homologues from macaque (88%), human (84%) and dog (82%). This sequence shows less than 75% homology to homologues from bovine, Xenopus, chicken, chimpanzee and zebrafish sources. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | Q61216 |
NCBI | 18202590 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Mre11 antibody, Double-strand break re Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to Mre11 by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein using Mre11 antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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