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Mouse TNF alpha protein

Catalog Number: orb80109

DispatchUsually dispatched within 2-3 weeks
$ 1,926.00
Catalog Numberorb80109
CategoryProteins
DescriptionMouse TNF alpha protein
Tested applicationsFA, HPLC, SDS-PAGE
Purity> 98.0% as determined by RP-HPLC and SDS-PAGE analysis
ConjugationUnconjugated
TargetMouse TNF alpha
Solubility (25°C)It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.1ng/ml, corresponding to Specific Activity of 10,000,000 Units/mg.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesLyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2.
Alternative namesAPC1 protein, Cachectin protein, DIF protein, Diff
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NoteFor research use only
Application notesExperiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of TNF-a as Reference Standard.
Expiration Date6 months from date of receipt.
Mouse TNF alpha protein

SDS-PAGE analysis of Mouse TNF alpha protein

  • Mouse Tnf protein [orb594857]

    Greater than 95% as determined by SDS-PAGE.

    16.4 kDa

    E.coli

    500 μg, 1 mg, 10 μg, 50 μg
  • Mouse TNF-alpha Protein, His Tag [orb1496307]

    Unconjugated

    95%

    19.1 kDa

    Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).

    1 mg, 100 μg
  • Mouse TNF Alpha/Tumor necrosis factor ELISA Kit [orb738503]

    Mouse

    15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum, plasma)

    < 1 pg/ml

    96 wells
  • Mouse ADAM17 / TACE / CD156b Protein [orb257936]

    Unconjugated

    90%

    63.4 kDa

    Mouse ADAM17, His Tag (orb257936) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8).

    500 μg, 50 μg
  • Mouse Lta protein [orb604233]

    Greater than 85% as determined by SDS-PAGE.

    23.6 kDa

    E.coli

    20 μg, 100 μg, 1 mg
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