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Catalog Number | orb80109 |
---|---|
Category | Proteins |
Description | Mouse TNF alpha protein |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and SDS-PAGE analysis |
Conjugation | Unconjugated |
Target | Mouse TNF alpha |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
Biological Activity | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.1ng/ml, corresponding to Specific Activity of 10,000,000 Units/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2. |
Alternative names | APC1 protein, Cachectin protein, DIF protein, Diff Read more... |
Note | For research use only |
Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of TNF-a as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse TNF alpha protein
Greater than 95% as determined by SDS-PAGE. | |
16.4 kDa | |
E.coli |
Unconjugated | |
95% | |
19.1 kDa | |
Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804). |
Mouse | |
15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum, plasma) | |
< 1 pg/ml |
Unconjugated | |
90% | |
63.4 kDa | |
Mouse ADAM17, His Tag (orb257936) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8). |
Greater than 85% as determined by SDS-PAGE. | |
23.6 kDa | |
E.coli |
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