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Catalog Number | orb1463635 |
---|---|
Category | Proteins |
Description | Recombinant Mouse M-CSF protein |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 85% by SDS-PAGE gel analyses |
MW | 38.7 KDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
UniProt ID | P07141 |
Source | E.Coli |
Expression System | 33-187 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
Alternative names | CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
81.8 kDa | |
Mouse M-CSF R, Fc Tag (orb383508) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948). |
Unconjugated | |
95% | |
57.1 kDa | |
Mouse M-CSF R, His Tag (orb383507) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-mFc is approximately 55-75 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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