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Catalog Number | orb80086 |
---|---|
Category | Proteins |
Description | Keratinocyte Growth Factor-1 Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa. The FGF-7 is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
Conjugation | Unconjugated |
Target | mKGF |
Solubility (25°C) | It is recommended to reconstitute the lyophilized KGF in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT. |
Biological Activity | The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is 10ng/ml corresponding to specific activity of 100,000 Units/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution containing 20 mM Phosphate buffer pH-8 and 0.1M NaCl. |
Alternative names | HBGF-7 protein, FGF7 protein, FGF-7 protein, KGF p Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.8 kDa | |
E.Coli |
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