Mouse Keratinocyte Growth Factor protein

• Catalog Number: orb80086
• Category: Proteins
• Description: Keratinocyte Growth Factor-1 Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa. The FGF-7 is purified by proprietary chromatographic techniques.
• Target: mKGF
• Conjugation: Unconjugated
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) solution containing 20 mM Phosphate buffer pH-8 and 0.1M NaCl.
• Purity: > 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
• Protein Sequence: MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT.
• Tested applications: FA, HPLC, SDS-PAGE
• Biological Origin: E.coli
• Biological Activity: The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is 10ng/ml corresponding to specific activity of 100,000 Units/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized KGF in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: HBGF-7 protein, FGF7 protein, FGF-7 protein, KGF p
• Note: For research use only