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Catalog Number | orb80068 |
---|---|
Category | Proteins |
Description | IL-10 Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18785 Dalton. The IL10 Mouse is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | mIL 10 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-10 Mouse Recombinant in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependant co-stimulation with IL-4 of mouse MC-9 cells was found to be 2ng/ml, corresponding to Specific Activity of x 100,000 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized after extensive dialysis against PBS. |
Alternative names | B-TCGF protein, CSIF protein, TGIF protein, IL-10 Read more... |
Note | For research use only |
Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.34 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-10 as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL10 protein
> 97% as determined by SDS-PAGE and HPLC. | |
18.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
18.9 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
> 95% as analyzed by SDS-PAGE and HPLC. | |
21-23 kDa, observed by reducing SDS-PAGE. | |
CHO |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |