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Mouse IL10 protein

Catalog Number: orb80068

DispatchUsually dispatched within 2-3 weeks
$ 3,328.00
Catalog Numberorb80068
CategoryProteins
DescriptionIL-10 Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18785 Dalton. The IL10 Mouse is purified by proprietary chromatographic techniques.
Tested applicationsFA, HPLC, SDS-PAGE
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
ConjugationUnconjugated
TargetmIL 10
Solubility (25°C)It is recommended to reconstitute the lyophilized IL-10 Mouse Recombinant in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.
Biological ActivityThe ED50 as determined by the dose-dependant co-stimulation with IL-4 of mouse MC-9 cells was found to be 2ng/ml, corresponding to Specific Activity of x 100,000 IU/mg.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe protein was lyophilized after extensive dialysis against PBS.
Alternative namesB-TCGF protein, CSIF protein, TGIF protein, IL-10
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NoteFor research use only
Application notesExperiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.34 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-10 as Reference Standard.
Expiration Date6 months from date of receipt.
Mouse IL10 protein

SDS-PAGE analysis of Mouse IL10 protein

  • Mouse IL10 protein (Active) [orb359015]

    > 97% as determined by SDS-PAGE and HPLC.

    18.7 kDa

    E.Coli

    10 μg, 100 μg, 500 μg
  • Mouse Il10 protein [orb594838]

    Greater than 95% as determined by SDS-PAGE.

    18.9 kDa

    E.coli

    50 μg, 500 μg, 1 mg, 10 μg
  • Mouse TIGIT Protein [orb348797]

    Unconjugated

    95%

    14.8 kDa

    Mouse TIGIT, His Tag (orb348797) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

    1 mg, 50 μg
  • Mouse TIGIT Protein [orb334938]

    Unconjugated

    95%

    39.5 kDa

    Mouse TIGIT, Fc Tag (orb334938) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

    1 mg, 50 μg
  • Mouse IL10 protein [orb391909]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    E. coli

    500 μg, 50 μg, 10 μg
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