Mouse IgG2b isotype control Phycoerythrin Antibody

SKU: orb343794

Description

Mouse IgG2b isotype control Phycoerythrin Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT, IF
• Dilution Range: IF: 1:1000-1:5000
• Application Notes:
  Mouse IgG2b isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG2b
• Clone No.: M2B
• Purity: This product was prepared from ascitic fluid by affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phycoerythrin conjugation Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control PE conjugated

Dot Blot of Mouse IgG2b Isotype Control Phycoerythrin Conjugated. Antigen: none. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Mouse IgG2b Isotype Control Phycoerythrin Conjugated blotted directly to membrane at above dilutions. Block: orb348637 for 60 min at RT.