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Mouse IgG2a Isotype Control (PE)

Catalog Number: orb222807

DispatchUsually dispatched within 5-10 working days
$ 355.00
Catalog Numberorb222807
CategoryAntibodies
DescriptionMouse IgG2a Isotype Control (PE)
Species/HostMouse
ClonalityMonoclonal
Clone NumberMOPC-173
Tested applicationsFC
IsotypeMouse IgG2a kappa
ImmunogenThe transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Concentration0.1 mg/ml
Dilution rangeNegative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
PurityPurified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationPE
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
NoteFor research use only
Application notesNegative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Expiration Date12 months from date of receipt.
Mouse IgG2a Isotype Control (PE)

Flow cytometric analysis of human peripheral blood using Mouse IgG2a Isotype Control (PE)

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