Mouse IgG1 antibody

• Catalog Number: orb198405
• Category: Antibodies
• Description: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ZX3
• Tested applications: FACS
• Reactivity: Mouse
• Isotype: IgG1
• Concentration: See vial for concentration
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P07750
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Alternative names: IgG1
• Note: For research use only
• Application notes: Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at ?633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Peripheral blood lymphocytes using Mouse IgG1 antibody