Mouse GCSF protein

• Catalog Number: orb80032
• Category: Proteins
• Description: Granulocyte MCSF Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14.2 kDa.GMCSF Mouse is purified by proprietary chromatographic techniques.
• Target: mGM CSF
• Conjugation: Unconjugated
• Buffer/Preservatives: GM-CSF Mouse was lyophilized with no additives.
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
• Tested applications: FA, HPLC, SDS-PAGE
• Application notes: < strong>Experiment Notes: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of GM-CSF as Reference Standard.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is 0.2 ng/ml, corresponding to Specific Activity of 5,000,000 IU/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20 mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pl
• Note: For research use only

SDS-PAGE analysis of Mouse GCSF protein