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Mouse Embryonic Fibroblast Whole Cell Lysate

Catalog Number: orb348722

Product Overview
Product Name Mouse Embryonic Fibroblast Whole Cell Lysate
Catalog Number orb348722
ReactivityMouse
Tested applicationsWB
Hazard InformationNon-Toxic
Target Mouse Embryonic Fibroblast Whole Cell Lysate
Product Properties
Form/Appearance Liquid: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
10% (v/v) Glycerol
Concentration 1.0 mg/ml
Storage Store vial at -70°C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Note For research use only.
Product Description

Mouse Embryonic Fibroblast Whole Cell Lysate

Application Notes
Application Notes ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95°C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 μl depending on the size format of your gel.
Validation Images
Western blot analysis of Mouse Embryonic Fibroblast (MEF) Whole Cell Lysate using Mouse Embryonic Fibroblast Whole Cell Lysate
Western blot analysis of Mouse Embryonic Fibroblast (MEF) Whole Cell Lysate using Mouse Embryonic Fibroblast Whole Cell Lysate
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