Mouse EGF protein

• Catalog Number: orb80014
• Category: Proteins
• Description: Mouse EGF protein
• Target: Mouse EGF
• Conjugation: Unconjugated
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) solution containing 1x PBS pH-7.4.
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human.
• MW: 6 kDa
• Tested applications: FA, HPLC, SDS-PAGE
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of EGF as Reference Standard.
• Biological Origin: E.coli
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 0.1 ng/ml.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Urogastrone protein, URG protein, EGF protein
• Note: For research use only