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Mouse Complement C5 Protein

Catalog Number: orb653662

DispatchUsually dispatched within 5-10 working days
$ 3,740.00
Catalog Numberorb653662
CategoryProteins
DescriptionDerived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
ReactivityMouse
TagC-10×His
Form/AppearancePowder
Purity95%
ConjugationUnconjugated
MW73.5 kDa (β chain) & 114.6 kDa (α chain)
TargetComplement C5
SourceMouse Complement C5, His Tag (orb653662) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1).
Expression SystemHEK293
Biological ActivityImmobilized Mouse Complement C5, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).
Expression RegionGln 19 - Glu 1680
Endotoxins1.0 EU per μg
Storage-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesComplement C5,C5,CPAMD4
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NoteFor research use only
Application notesThis protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Mouse Complement C5 Protein

Immobilized Mouse Complement C5, His Tag (Cat. No. orb653662) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).

  • Mouse Complement C5a Protein [orb570259]

    Unconjugated

    95%

    10.9 kDa

    Mouse Complement C5a, His Tag (orb570259) is expressed from E. coli cells. It contains AA Asn 679 - Arg 755 (Accession # P06684).

    100 μg, 1 mg
  • Mouse Properdin Protein [orb638749]

    Unconjugated

    95%

    49.8 kDa

    Mouse Properdin, His Tag (orb638749) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Pro 464 (Accession # P11680-1).

    100 μg, 1 mg
  • Mouse C5 ELISA Kit [orb776808]

    Mouse

    0.16-10 ng/mL

    0.073 ng/mL

    48 Test, 24 t, 96 Test
  • Mouse C5 protein [orb391326]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing RP-HPLC and reducing SDS-PAGE.

    E. coli

    500 μg, 50 μg, 10 μg
  • Mouse Complement Component C5a ELISA KIT [orb1086277]

    Mouse

    15.60-1000 pmol/l

    7 pmol/l

    96 Test
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