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Catalog Number | orb653662 |
---|---|
Category | Proteins |
Description | Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 73.5 kDa (β chain) & 114.6 kDa (α chain) |
Target | Complement C5 |
Source | Mouse Complement C5, His Tag (orb653662) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Mouse Complement C5, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested). |
Expression Region | Gln 19 - Glu 1680 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Complement C5,C5,CPAMD4 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Mouse Complement C5, His Tag (Cat. No. orb653662) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).
Unconjugated | |
95% | |
10.9 kDa | |
Mouse Complement C5a, His Tag (orb570259) is expressed from E. coli cells. It contains AA Asn 679 - Arg 755 (Accession # P06684). |
Unconjugated | |
95% | |
49.8 kDa | |
Mouse Properdin, His Tag (orb638749) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Pro 464 (Accession # P11680-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing RP-HPLC and reducing SDS-PAGE. | |
E. coli |
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