Mouse anti-HLA-DR Monoclonal Antibody

• Catalog Number: orb1520114
• Category: Antibodies
• Description: HLA-DR Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LN3
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Immunogen: Activated human peripheral blood mononuclear cells
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1000; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HLA-DR
• UniProt ID: P01903
• NCBI: NP_061984.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: MHC class II antigen DRA; HLA-DRA1; histocompatibi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 µg) from KG-1, SNB-75, Raji, Ramos, and SR (10 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG.

Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (orb1520114) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG.

Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520114) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).