MMP9 antibody

• Catalog Number: orb11064
• Category: Antibodies
• Description: MMP9 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MMP9 (611-707/707 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: MMP9
• UniProt ID: P14780
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Matrix metalloproteinase-9 precursor; MMP-9; MMP9;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Møller, Henrik Devitt et al. Role of fibulin-5 in metastatic organ colonization Mol Cancer Res, 9, 553-563 (2011)

PubMed: 21454378

Applications

IHC

Reactivity

Mouse

Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)

PubMed: 36278554

Applications

IHC

Reactivity

Human

Immunohistochemical staining of paraffin embedded human stomach cancer tissue using MMP9 antibody (1:100, heat retrieval)

Immunofluorescence: IF image of human endometrial epithelial cells grown in 3D (1:100)

IHC-P of human stomach cancer tissue using MMP9 antibody (orb11064 at 1:200)

Western blot of mouse cell lysates using MMP9 antibody (1:1000, 5% milk, WB)

Paraffin-embedded rat ovary carcinoma tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).MMP9 antibody at 1:200 dilution with overnight incubation period

Western Blot on cells and tissues using MMP9 antibody (working concentration: 2 ug/ml)

Immunohistochemical analysis of formalin fixed and paraffin embedded human colon carcinoma using MMP9 antibody