MHC Class II antibody

• Catalog Number: orb101661
• Category: Antibodies
• Description: MHC Class II antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Canine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MHC Class II (201-296/296 aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: MHC Class II
• UniProt ID: P28068
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: HLA-DMB; HLA-DMβ; HLA DMβ; DMA; DMB; DP beta 1; DP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Tavares Pereira, Miguel et al. Selected Uterine Immune Events Associated With the Establishment of Pregnancy in the Dog Front Vet Sci, 7, 625921 (2020)

PubMed: 33634180

Nowak, Marta et al. Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function PLoS One, 13, e0191374 (2018)

PubMed: 29364921

Nowaczyk, Renata M. et al. Cells expressing CD4, CD8, MHCII and endoglin in the canine corpus luteum of pregnancy, and prepartum activation of the luteal TNFα system Theriogenology, 98, 123-132 (2017)

PubMed: 28601149

Paraffin-embedded human pancreatic cancer tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min). MHC Class II antibody at 1:200 dilution with overnight incubation.

IHC-P of human pancreatic cancer tissue (MHC Class II antibody at 1:200)