MEPCE Antibody

• Catalog Number: orb1247270
• Category: Antibodies
• Description: MEPCE Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: KQQRKFQYGNYCK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human for this particular batch.Immunohistochemistry:In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MEPCE
• UniProt ID: Q7L2J0
• NCBI: NP_062552.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human for this particular batch.Immunohistochemistry:In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247270 (1 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247270 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.