MEKK1 (phospho-T1402) antibody

• Catalog Number: orb256669
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MEKK1 (phospho-T1402)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T1402 of human MEKK1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAP3K1
• Entrez: 116667, 4214
• UniProt ID: Q13233, P53349, Q62925
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MAPKKK1 antibody, anti MEKK antibody, anti ME
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MEKK1 (Phospho-T1402) expression in HeLa (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 164 kD; Observed band size: 195 kD)

Immunohistochemical analysis of MEKK1 (Phospho-T1402) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.