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Product Overview
Product Name MCF-7 Cell Nuclear Extract
Catalog Number orb348687
Tested applicationsWB
Hazard InformationNon-Toxic
Target MCF-7 Cell Nuclear Extract
Product Properties
Form/Appearance Liquid: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
10% (v/v) Glycerol
Concentration 1.0 mg/ml
Storage Store vial at -70°C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Note For research use only.
Product Description

MCF-7 Cell Nuclear Extract

Application Notes
Application Notes ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95°C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 l depending on the size format of your gel.
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