MBD4 Antibody

• Catalog Number: orb99204
• Category: Antibodies
• Description: MBD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4 (566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 66051 MW
• UniProt ID: O95243
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Methyl-CpG-binding domain protein 4;3.2.2.-;Methyl
• Note: For research use only
• Application notes: WB: The detection limit for MBD4 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: MCF-7 Cell Lysate, Lane 3: HELA Cell Lysate using MBD4 antibody

Western blot analysis of Lane 1: Recombinant Human MBD4 Protein 10ng, Lane 2: Recombinant Human MBD4 Protein 5ng, Lane 3: Recombinant Human MBD4 Protein 2.5ng using MBD4 antibody

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: A549 Cell Lysate, Lane 4: HELA Cell Lysate, Lane 5: MCF-7 Cell Lysate using MBD4 antibody