MAPT antibody

• Catalog Number: orb216148
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TAU (Phospho-S713)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S713 of human TAU protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPT
• Entrez: 17762, 4137
• UniProt ID: P10636, P19332, P10637
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MAPTL antibody, anti MTBT1 antibody, anti TAU
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TAU (Phospho-S713) expression in mouse brain (A), rat brain (B), HepG2 (C), MCF7 (D), HEK293T (E) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 50 kD)

Immunofluorescent analysis of TAU (Phospho-S713) staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).