MAP4K1 Antibody

• Catalog Number: orb1247946
• Category: Antibodies
• Description: MAP4K1 Antibody
• Target: MAP4K1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: DVVDPDIFNRDPR-C
• UniProt ID: Q92918
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 2.5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAP4K1, mitogen-activated protein kinase kinase ki
• Note: For research use only
• NCBI: NP_009112.1, NP_001036065.1
• Expiration Date: 12 months from date of receipt.

orb1247946 (2.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247946 staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.