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LPIN3 antibody

Catalog Number: orb19353

DispatchUsually dispatched within 5-10 working days
$ 502.00
Catalog Numberorb19353
CategoryAntibodies
DescriptionGoat polyclonal antibody to LPIN3
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC
ReactivityHuman
Dilution rangeELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2-3µg/ml
ConjugationUnconjugated
MW93.6
TargetLipin 3
Entrez64900
Protein SequenceKPKQKEDAVATD
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti LPIN3 antibody, anti lipin 3 antibody, anti R
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Expiration Date12 months from date of receipt.
LPIN3 antibody

orb19353 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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    Mouse

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