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Product Overview
Product Name LPIN3 antibody
Catalog Number orb19353
Species/HostGoat
ReactivityHuman
ConjugationUnconjugated
Tested applicationsELISA, IHC-P
Immunogen C-KPKQKEDAVATD (851 AA)
Target LPIN3
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 80 kDa
NCBI NP_075047.1
Entrez 64900
Product Description

Goat polyclonal antibody to LPIN3

Application Notes
Dilution Range ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2-3µg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.

IHC: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3ug/ml.

WB: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Validation Images
orb19353 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb19353 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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