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Catalog Number | orb2292656 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant LGALS1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1E8-1B2 |
Tested applications | ELISA, IF, IP, WB |
Reactivity | Human |
Isotype | IgG2b Lambda |
Immunogen | LGALS1 (AAH01693, 1 a.a. ~ 135 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD |
NCBI | AAH01693 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged LGALS1 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to LGALS1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunoprecipitation of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 MaxPab rabbit polyclonal antibody.
LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Western Blot analysis of LGALS1 expression in HepG2.
Western Blot analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2. Lane 1: LGALS1 transfected lysate (14.7 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (40.59 KDa).