LEP Antibody (N-term)

• Catalog Number: orb1934701
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB36463
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 18641 Da
• Target: This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human LEP.
• UniProt ID: P41159
• NCBI: NP_000221.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leptin, Obese protein, Obesity factor, LEP, OB, OB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the LEP antibody detected the LEP protein (arrow).

Confocal immunofluorescent analysis of LEP Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.

LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.