LDHB Antibody

• Catalog Number: orb1248229
• Category: Antibodies
• Description: LDHB Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EEATVPNNKIT
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 35kDa
• Target: LDHB
• UniProt ID: P07195
• NCBI: NP_002291.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in Human Heart and Kidney lysates (calculated MW of 36.6kDa according to NP_002291.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248229 (0.3 ug/ml) staining of Human Heart (A) and Human Kidney (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248229 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.