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KRT7 Antibody / Cytokeratin 7

Catalog Number: orb749625

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749625
CategoryAntibodies
DescriptionIt recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenRecombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
UniProt IDP08729
Tested applicationsFACS, IF, IHC-P, WB
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesOptimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberKRT7/760
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis Cytokeratin 7 antibody is available for research use only.
NoteFor research use only
KRT7 Antibody / Cytokeratin 7

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

KRT7 Antibody / Cytokeratin 7

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

KRT7 Antibody / Cytokeratin 7

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

KRT7 Antibody / Cytokeratin 7

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone KRT7/760). Predicted molecular weight ~51 kDa.

KRT7 Antibody / Cytokeratin 7

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

KRT7 Antibody / Cytokeratin 7

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760, green) and Reddot nuclear stain (red).

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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg