KRT12 Antibody

• Catalog Number: orb1269415
• Category: Antibodies
• Description: KRT12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: KRT12
• UniProt ID: Q99456
• NCBI: Q99456
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Keratin, type I cytoskeletal 12, Cytokeratin-12, C
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H + L) (1583138) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:500 dilution + Y79 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis in K562 cell line lysates (35 ug/lane).

KRT12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of KRT12 Antibdy for immunohistochemistry.