Kcnj11 antibody

• Catalog Number: orb19764
• Category: Antibodies
• Description: Goat polyclonal antibody to Kcnj11
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 43.6
• Target: Kcnj11 / Kir6.2
• Entrez: 3767
• Protein Sequence: ERRARFVSKKGNC
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KCNJ11 antibody, anti Kir6.2 antibody, anti p
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5ug/ml.WB: Approx 45kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.6kDa according to NP_034732.1). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

References

Shimbo, Tomonori et al. In vitro effect of nicorandil on the carbachol-induced contraction of the lower esophageal sphincter of the rat J Pharmacol Sci, 131, 267-274 (2016)

PubMed: 27562702

orb19764 (0.01ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19764 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.