JAZF1 Antibody

• Catalog Number: orb2635171
• Category: Antibodies
• Description: JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 1-130 was used as the immunogen for the JAZF1 antibody.
• UniProt ID: Q86VZ6
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the JAZF1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-JAZF1-1C2
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This JAZF1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human testis tissue with JAZF1 antibody (clone PCRP-JAZF1-1C2) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells using JAZF1 antibody (green, clone PCRP-JAZF1-1C2) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with JAZF1 antibody (blue, clone PCRP-JAZF1-1C2), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free JAZF1 antibody (clone PCRP-JAZF1-1C2) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using JAZF1 antibody (clone PCRP-JAZF1-1C2). These results demonstrate the foremost specificity of the PCRP-JAZF1-1C2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.