Irs4 Antibody

• Catalog Number: orb1248556
• Category: Antibodies
• Description: Irs4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-DRGTKERKEAKEVRD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799.Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 150kDa
• Target: Irs4
• UniProt ID: Q9Z0Y7
• NCBI: NP_034702.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Irs4, insulin receptor substrate 4, IRS-4, RP23-41
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799.Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089.
• Expiration Date: 12 months from date of receipt.

orb1248556 (0.2 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.