IRF6 antibody

• Catalog Number: orb18656
• Category: Antibodies
• Description: Goat polyclonal antibody to IRF6
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.2-0.6 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 53.1
• Target: IRF6
• Entrez: 3664
• Protein Sequence: TPSMQLPPALPPQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti IRF6 antibody, anti interferon regulatory fac
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration, 2-3ug/ml.WB: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6 μg/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing IRF6 (RC201579) and probed with orb18656 (mock transfection in first lane), tested by Origene.

orb18656 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Human Ovary lysate using IRF6 antibody

Immunohistochemical staining of Human Prostate using IRF6 antibody