INos/Nos-2 Rabbit Polyclonal Antibody

• Catalog Number: orb500991
• Category: Antibodies
• Description: INos/Nos-2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse, Rabbit
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human iNos/Nos-2 (1051-1153/1153aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: NOS2
• UniProt ID: P35228
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: i NOS; Nitric Oxide Synthase, Inducible; HEP NOS;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-iNOS antibody (orb500991), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (orb500991) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (orb500991) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: DU145 Cell (Human) Lysate at 40 ug, Primary: Anti-iNos/Nos-2 (orb500991) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130kD, Observed band size: 125kD.

Sample: MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-iNos/Nos-2 (orb500991) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130kD, Observed band size: 125kD.