TSPO Antibody

• Catalog Number: orb1246823
• Category: Antibodies
• Description: TSPO Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RDNHGWRGGRRLPE
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TSPO
• UniProt ID: P30536
• NCBI: NP_000705.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TSPO, PBR, translocator protein (18kDa) , BZRP, DB
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Breast Cancer lysates (calculated MW of 18.8kDa according to NP_000705.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Colon shows strong cytoplasm staining of absorptive cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246823 (0.1 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246823 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246823 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.