TRIM28 Antibody (N-term)

• Catalog Number: orb1937899
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB09760
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 88550 Da
• Target: This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
• UniProt ID: Q13263
• NCBI: NP_005753.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription intermediary factor 1-beta, TIF1-bet
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-TRIM28 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: PC-3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.

TRIM28 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

TRIM28 Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).