TRAK1 Rabbit Polyclonal Antibody

• Catalog Number: orb582367
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TRAK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRAK1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 106 kDa
• Target: TRAK1
• UniProt ID: Q9UPV9
• Protein Sequence: Synthetic peptide located within the following region: ILETEAADLGNDERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFF
• NCBI: NP_001036111
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: DEE68, MILT1, EIEE68, OIP106
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Isoforms containing the peptide sequence are present at ~77 and ~64 kDa.

Sample Type: HCT15, Antibody dilution: 1.0 ug/ml. There is BioGPS gene expression data showing that TRAK1 is expressed in HCT15.

Sample Type: Hela, Antibody dilution: 1.0 ug/ml. TRAK1 is supported by BioGPS gene expression data to be expressed in HeLa.

Sample Type: HepG2, Antibody dilution: 1.0 ug/ml. TRAK1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.

Sample Type: HT1080, Antibody dilution: 1.0 ug/ml. There is BioGPS gene expression data showing that TRAK1 is expressed in HT1080.

Positive control (+): HepG2 Cell Lysate (HG), Negative control (-): Human Liver Tumor (T-LI), Antibody concentration: 1 ug/ml.

Lanes: Lane 1: 10 ug myc-hTRAK1 transfected HEK293 lysate, Primary Antibody dilution: 1:500, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody dilution: 1:2000, Gene Name: TRAK1.

WB Suggested Anti-TRAK1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: HT1080 cell lysate. There is BioGPS gene expression data showing that TRAK1 is expressed in HT1080.