Rat Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit

• Catalog Number: orb1088229
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1(KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1(KEAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: P57790
• Sensitivity: 0.057 ng/mL
• Alternative names: INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KEAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KEAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KEAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KEAP1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.