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Catalog Number | orb1519912 |
---|---|
Category | Antibodies |
Description | VISTA Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR035F |
Tested applications | FC, ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 261 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 100 µg/ml |
Dilution range | WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | VISTA |
UniProt ID | Q9H7M9 |
NCBI | NP_071436.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | PP2135; V-set immunoregulatory receptor; V-set dom Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (orb1519912) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |