Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody

• Catalog Number: orb1530835
• Category: Antibodies
• Description: ECS (DYKDDDDK) Tag Antibody
• Target: ECS (DYKDDDDK) Tag
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Form/Appearance: Whole IgG
• Concentration: 100 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Enterokinase Cleavage Site (DYKDDDDK)
• Tested applications: ELISA, ICC, IP, WB
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; ICC - 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:75,000; for coating plates 1:100 - 1:500
• Application notes: Format: Whole IgG
• Antibody Type: Recombinant Antibody
• Clone Number: BLRE00G
• Storage: 2 - 8°C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of ECS (DYKDDDDK)-tagged protein by WB from non-transfected HEK293 cells, HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1, IDO1-ECS(DYKDDDDK)-HIS (Internal Tag), IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag), and SUP-M2 cells, at 1:1000.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag) lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1 lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing ECS (DYKDDDDK)-tagged nuclear protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing an ECS (DYKDDDDK)-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.