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Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD20266 |
| Immunogen | This P21Cip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-140 amino acids from human P21Cip1. Antigen Region: 105-140 aa. |
| Target | CDKN1A (HGNC:1784) |
| Molecular Weight | 18119 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of P21Cip1-S130 antibody in Hela cell line lysates (35 ug/lane). P21Cip1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of P21 Antibody (WAF1) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000380.1, NP_001207707.1, NP_001207706.1, NP_510867.1 |
|---|
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Protocol Information
P21Cip1 Antibody (orb1925227)
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