KRT19 Antibody / Cytokeratin 19

SKU: orb750301

Description

Cytokeratin-19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SPM561
• Immunogen: Human mammary epithelial organoids were used as the immunogen for this Cytokeratin 19 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (clone SPM561).

Western blot testing of human MCF7 cells with Cytokeratin 19 antibody (clone SPM561). Predicted molecular weight ~43 kDa.

Flow cytometry testing of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561); Red=isotype control, Blue= Cytokeratin 19 antibody.

Quick Database Links

UniProt

P08727