ILK Antibody (S246)

• Catalog Number: orb1929025
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13868
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 51419 Da
• Target: This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK.
• UniProt ID: Q13418
• NCBI: NP_001265370.1, NP_001014795.1, NP_004508.1, NP_001014794.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Integrin-linked protein kinase, 59 kDa serine/thre
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35 ug/lane). ILK (arrow) was detected using the purified Pab.

Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.