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Catalog Number | orb1671705 |
---|---|
Category | Antibodies |
Description | IL-17F Antibody [8F5.1A9], Rabbit IgG |
Clonality | Recombinant |
Clone Number | 8F5.1A9 |
Tested applications | FC |
Reactivity | Mouse |
Isotype | IgG kappa |
Immunogen | This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | Il17f |
UniProt ID | Q7TNI7 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Interleukin-17F, IL17F Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 8F5.1A9 (orb1671705 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.