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IL-17F Antibody [8F5.1A9], Rabbit IgG

Catalog Number: orb1671705

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$ 1,050.00
Catalog Numberorb1671705
CategoryAntibodies
DescriptionIL-17F Antibody [8F5.1A9], Rabbit IgG
ClonalityRecombinant
Clone Number8F5.1A9
Tested applicationsFC
ReactivityMouse
IsotypeIgG kappa
ImmunogenThis antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
Antibody TypePrimary Antibody
Concentrationbatch dependent
ConjugationUnconjugated
TargetIl17f
UniProt IDQ7TNI7
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namesInterleukin-17F, IL17F
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NoteFor research use only
Expiration Date12 months from date of receipt.
IL-17F Antibody [8F5.1A9], Rabbit IgG

Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 8F5.1A9 (orb1671705 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.